110824 UVM-Listeria selective enrichment broth modified for microbiology GranuCult®
Simpan pada +15°C hingga +25°C.
Appearance (clearness) | clear to turbid |
Appearance (color) | yellowish-brown |
pH-value (25 °C) | 7.0 - 7.4 |
Growth promotion test in accordance with the current version of DIN EN ISO 11133. | |
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)) | ≤ 100 |
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)) | ≤ 100 |
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | ≥ 10000 |
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)) | ≥ 10000 |
Growth (Listeria monocytogenes ATCC 35152 (WDCM 00109)) | > 10 colonies on ALOA medium, blue green with opaque halo |
Growth (Listeria monocytogenes ATCC 13932 (WDCM 00021)) | > 10 colonies on ALOA medium, blue green with opaque halo |
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | < 100 colonies on TSA |
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)) | < 100 colonies on TSA |
Mixed inoculum of Listeria and S.aureus Additionaly monoculture of S.aureus and B.cereus Confirmation on ALOA medium resp. Tryptic soy agar Incubation: 20 - 26 hours at 30 ± 2°C, aerobic Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C |