| Spending Type | : Consumable |
| Category | : Microbiology and Life Science |
| Type | : Culture Media |
| SKU | : 1.00550.5000 |
| Package | : 5 kg |
| Packing | : Drum plastik |
Media kultur dehydrated dengan vegetabel peptone, Tryptic Soy Broth (TSB), dapat digunakan sebagai alternatif pilihan untuk mengganti penggunaan Soybean Casein Digest Medium (SCDM). Media ini sudah melalui filtrasi dan iradiasi dengan sinar gamma untuk proses validasi mikrobiologi dari proses filling/pengisian yang aseptis (Pengujian media fill).
Tryptic Soy Broth ini cocok digunakan dalam pengujian media fill karena sesuai dengan rekomendasi FDA Aseptic Guide untuk produk-produk yang aseptis. Penggunaan bahan bahan non hewan dapat minimalisir potensi kontaminasi.
Formulasi media basal (Vegetable Peptone Broth) dilakukan sesuai dengan rekomendasi standar Eropa, Jepang dan United States Pharmacopoeia (EP, 2.6.12.; JP, 5.05 dan USP 16).
| INFORMASI | KETERANGAN |
| Komposisi | Peptone Non-Animal Origin 20 g/l D(+)-Glucose Monohydrate 2.5 g/l NaCl 5.0 g/l K2HPO4 2.5 g/l |
| pH | 7.1 - 7.5 pada suhu 25 °C |
| Appearance (clearness) | clear |
| Warna | Coklat kekuningan |
| Stability test (7 days, room temperature) | clear |
| Sterility - 2 weeks at 20-25 °C |
no growth |
| - 2 weeks at 30-35 °C | no growth |
| mycoplasma (qPCR) | negative post sterility testing |
| Minimum irridation dose | ≥ 48 kGy |
| Maximum irridation dose | ≤ 76 kGy |
| Growth promotion test in accordance with the harmonised method of EP and USP | |
| Inoculum on reference medium - Escherichia coli ATCC 8739 (WDCM 00012) |
10 - 100 |
| - Staphylococcus aureus ATCC 6538 (WDCM 00032) | 10 - 100 |
| - Staphylococcus aureus ATCC 25923 (WDCM 00034) | 10 - 100 |
| - Staphylococcus epidermidis ATCC 12228 (WDCM 00036) | 10 - 100 |
| - Streptococcus pneumoniae ATCC 6301 | 10 - 100 |
| - Bacillus subtilis ATCC 6633 (WDCM 00003) | 10 - 100 |
| - Candida albicans ATCC 2091 (WDCM 00055) | 10 - 100 |
| - Candida albicans ATCC 10231 (WDCM 00054) | 10 - 100 |
| - Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053) | 10 - 100 |
| - Pseudomonas aeruginosa ATCC 9027 (WDCM 00026) | 10 - 100 |
| - Salmonella typhimurium ATCC 14028 (WDCM 00031) | 10 - 100 |
| Reference medium: Tryptic Soy Agar (Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus epidermidis), SABOURAUD-2%-Glucose Agar (Candida albicans, Aspergillus brasiliensis) |
|
| Growth / 24h at 30-35°C - Escherichia coli ATCC 8739 (WDCM 00012) |
visible growth |
| - Staphylococcus aureus ATCC 6538 (WDCM 00032) | visible growth |
| - Streptococcus pneumoniae ATCC 6301 | visible growth |
| - Bacillus subtilis ATCC 6633 (WDCM 00003) | visible growth |
| - Pseudomonas aeruginosa ATCC 9027 (WDCM 00026) | visible growth |
| - Salmonella typhimurium ATCC 14028 (WDCM 00031) | visible growth |
| Growth / 3 days at 20-25°C - Staphylococcus aureus ATCC 6538 (WDCM 00032) |
visible growth |
| - Staphylococcus aureus ATCC 25923 (WDCM 00034) | visible growth |
| - Staphylococcus epidermidis ATCC 12228 (WDCM 00036) | visible growth |
| - Bacillus subtilis ATCC 6633 (WDCM 00003) | visible growth |
| Growth / 5 days at 20-25°C - Candida albicans ATCC 2091 (WDCM 00055) |
visible growth |
| - Candida albicans ATCC 10231 (WDCM 00054) | visible growth |
| - Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053) | visible growth |
| Penyimpanan | Simpan pada suhu 15°C - 25°C |
