Media lactose broth digunakan untuk mengkultur Salmonella dan bakteri koliform lainnya dari makanan, olahan susu, serta minuman. Beberapa informasi teknis yang harus diperhatikan sebelum penggunaan antara lain:
INFORMASI | KETERANGAN |
Appearance (clearness) | clear |
Appearance (color) | yellowish |
pH-value (25 °C) | 6.7 - 7.1 |
Growth promotion test in accordance with the current version of DIN EN ISO 11133. | |
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≤ 100 |
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≤ 100 |
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | ≤ 100 |
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | ≤ 100 |
Inoculum on reference medium (Salmonella diarizonae ATCC 12325) | ≤ 100 |
Inoculum on reference medium (Salmonella diarizonae ATCC 29934) | ≤ 100 |
Inoculum on reference medium (Salmonella abortivoequina ATCC 9842) | ≤ 100 |
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | ≤ 100 |
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | ≤ 100 |
Growth (Escherichia coli ATCC 8739 (WDCM 00012)) | good to very good |
Growth Escherichia coli ATCC 25922 (WDCM 00013) | good to very good |
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | good to very good |
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | good to very good |
Growth (Salmonella diarizonae ATCC 12325) | good to very good |
Growth (Salmonella diarizonae ATCC 29934) | good to very good |
Growth (Salmonella abortivoequina ATCC 9842) | good to very good |
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | good to very good |
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | good to very good |
Gas (Escherichia coli ATCC 8739 (WDCM 00012)) | + |
Gas (Escherichia coli ATCC 25922 (WDCM 00013)) | + |
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | - |
Gas (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | - |
Gas (Salmonella diarizonae ATCC 12325) | - |
Gas (Salmonella diarizonae ATCC 29934) | - |
Gas (Salmonella abortivoequina ATCC 9842) | - |
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | - |
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | - |
Inkubasi | 24°C - 35°C, aerob |
Penyimpanan | Simpan pada suhu 15 °C - 25°C |
Simpan pada +15°C hingga +25°C