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BOLTON BROTH SELECTIVE SUPPLEMENT ACC. T

Merck BOLTON BROTH SELECTIVE SUPPLEMENT ACC. T

Spending Type : Consumable
Category : Microbiology and Life Science
Type : Culture Media
SKU : 1.00079.0010
Package : 10 x 1 vial
Packing : Peti/Kotak fiber

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Deskripsi Merck BOLTON BROTH SELECTIVE SUPPLEMENT ACC. T

100079 Suplemen Selektif Kaldu Bolton menurut ISO 10272-1




Simpan pada +2°C hingga +8°C.

Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004)

10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005)10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156)10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023)≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004)

> 10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005)> 10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156)> 10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013)

no growth
- Escherichia coli ATCC 8739 (WDCM 00012)no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023)no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004)

greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005)greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156)greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

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