100079 Suplemen Selektif Kaldu Bolton menurut ISO 10272-1
Simpan pada +2°C hingga +8°C.
Growth promotion test according to ISO 11133 Mixed inoculum of Campylobacter, E.coli and P.mirabilis. Additionaly monoculture of E.coli and P.mirabilis. Afterwards confirmation on mCCDA resp. Tryptic soy agar | |
Inoculum on reference medium - Campylobacter coli ATCC 43478 (WDCM 00004) | 10 - 100 |
- Campylobacter jejuni ATCC 33291 (WDCM 00005) | 10 - 100 |
- Campylobacter jejuni ATCC 29428 (WDCM 00156) | 10 - 100 |
- Escherichia coli ATCC 25922 (WDCM 00013) | ≥ 1000 |
- Escherichia coli ATCC 8739 (WDCM 00012) | ≥ 1000 |
- Proteus mirabilis ATCC 29906 (WDCM 00023) | ≥ 1000 |
Growth on mCCDA - Campylobacter coli ATCC 43478 (WDCM 00004) | > 10 CFU |
- Campylobacter jejuni ATCC 33291 (WDCM 00005) | > 10 CFU |
- Campylobacter jejuni ATCC 29428 (WDCM 00156) | > 10 CFU |
Growth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013) | no growth |
- Escherichia coli ATCC 8739 (WDCM 00012) | no growth |
- Proteus mirabilis ATCC 29906 (WDCM 00023) | no growth |
Colony colour - Campylobacter coli ATCC 43478 (WDCM 00004) | greyish |
- Campylobacter jejuni ATCC 33291 (WDCM 00005) | greyish |
- Campylobacter jejuni ATCC 29428 (WDCM 00156) | greyish |
Incubation: Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic |