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BOLTON broth (base) 500 G

Merck BOLTON broth (base) 500 G

Spending Type : Consumable
Category : Microbiology and Life Science
Type : Culture Media
SKU : 1.00068.0500
Package : 500 g
Packing : Botol plastik

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Deskripsi Merck BOLTON broth (base) 500 G

100068 Bolton Selective Enrichment Broth (Base) untuk mikrobiologi GranuCult®

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.


Simpan pada +15°C hingga +25°C.

Appearance (color)dark red to black
pH-value (25 °C)7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar.
Inoculum on reference medium (Campylobacter coli ATCC 43478 (WDCM 00004))≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 33291 (WDCM 00005))≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 29428 (WDCM 00156))≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 10000
Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023))≥ 10000
Growth on mCCDA (Campylobacter coli ATCC 43478 (WDCM 00004))> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 33291 (WDCM 00005))> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 29428 (WDCM 00156))> 10 CFU
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))no growth
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))no growth
Growth on Tryptic soy agar (Proteus mirabilis ATCC 29906 (WDCM 00023))no growth
Colony color (Campylobacter coli ATCC 43478 (WDCM 00004))greyish
Colony color (Campylobacter jejuni ATCC 33291 (WDCM 00005))greyish
Colony color (Campylobacter jejuni ATCC 29428 (WDCM 00156))greyish
Incubation:
Bolton broth: 5 ± 1 hours at 37 ± 1 °C, afterwards 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
Confirmation on mCCDA: at least 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C, aerobic

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