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LUGOL'S SOLUTION (DILUTED IODINE-POTASS

Merck LUGOL'S SOLUTION (DILUTED IODINE-POTASS

Spending Type : Consumable
Category : Chemical Reagent
Type : Microscopy and Diagnostic
SKU : 1.09261.1000

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Deskripsi Merck LUGOL'S SOLUTION (DILUTED IODINE-POTASS

109261 larutan Lugol (diencerkan dengan larutan iodine-potassium iodide ) Used to form a crystal violet dye-iodine complex in Gram staining

atau disebut juga : Iodine-potassium iodide solution

In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.
Used to form a crystal violet dye-iodine complex in Gram staining

Simpan pada +15°C hingga +25°C.

Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red